Yonaha-Nagato F, Sumida T
Division of Dermatology, Sakura Hospital, Toho University School of Medicine.
Nihon Rinsho. 1995 Oct;53(10):2473-8.
To address the question of whether the human T cell leukemia virus type I (HTLV-I) gene is associated with the etiology of Sjögren's syndrome (SS), RNA expression of HTLV-I gag, pol, env, and tax genes in labial salivary glands (LSGs) from SS patients who were seronegative for antibodies to HTLV-I was examined using RT-PCR method. The HTLV-I tax gene, but not the HTLV-I gag, pol, or env genes, was detected in LSG samples from 4 of 14 patients (29%). The nucleotide sequences of the HTLV-I pXIV region in these 4 patients' LSGs showed 100% homology to the HTLV-I pXIV gene from the MT-2 cell line. In conclusion, these findings suggest that products encoding sequences homologous to the HTLV-I pXIV gene in SS patients' LSGs might be candidates for self-antigen and/or lead to activation of autoreactive T lymphocytes through trans-acting transcriptional activation.
为了探讨人类I型嗜T细胞淋巴瘤病毒（HTLV-I）基因是否与干燥综合征（SS）的病因相关，我们采用逆转录聚合酶链反应（RT-PCR）方法检测了HTLV-I抗体血清学阴性的SS患者唇腺（LSG）中HTLV-I gag、pol、env和tax基因的RNA表达。在14例患者中的4例（29%）的LSG样本中检测到了HTLV-I tax基因，但未检测到HTLV-I gag、pol或env基因。这4例患者LSG中HTLV-I pXIV区域的核苷酸序列与MT-2细胞系的HTLV-I pXIV基因显示出100%的同源性。总之，这些发现表明，SS患者LSG中编码与HTLV-I pXIV基因同源序列的产物可能是自身抗原的候选物和/或通过反式转录激活导致自身反应性T淋巴细胞的激活。
