Macchia T, Mancinelli R, Gentili S, Lugaresi E C, Raponi A, Taggi F
Clinical Biochemistry Department, Istituto Superiore di Sanitá, Roma, Italy.
J Anal Toxicol. 1995 Jul-Aug;19(4):241-6. doi: 10.1093/jat/19.4.241.
The present study evaluates the suitability of headspace gas chromatography (GC) with a capillary column as a method for determining the ethanol content in different biological fluids. This procedure allows the use of headspace GC not only as a reference method but also in routine diagnostics and monitoring work. The recent literature reviewed reports no standardized methodology that is at the same time suitable for ethanol determination in all routinely available biological fluids (blood, serum, plasma, urine, and saliva). The proposed procedure seems to be a good solution to the problem. The reproducibility study for the biological fluids tested resulted in coefficients of variation that ranged from 0.8 to 2.9% and recoveries that averaged 99%. Linearity was verified in the range of 0.01-20 g/L of ethanol in aqueous solutions. Sensitivity was determined to be 0.01 g/L. Ethanol measurement by this method is easy, simple, and highly reliable, and only a small sample volume (0.1 mL) is required. An internal standard and sample manipulation are not necessary. The obtained results suggest that the use of headspace GC could be extended from confirmatory analyses to routine application in many biomedical fields.
本研究评估了配备毛细管柱的顶空气相色谱法（GC）作为测定不同生物流体中乙醇含量方法的适用性。该程序不仅允许将顶空气相色谱法用作参考方法，还可用于常规诊断和监测工作。最近回顾的文献报道，尚无一种标准化方法能同时适用于所有常规可得生物流体（血液、血清、血浆、尿液和唾液）中的乙醇测定。所提出的程序似乎是解决该问题的一个良好方案。对测试的生物流体进行的重现性研究得出变异系数在0.8%至2.9%之间，回收率平均为99%。在水溶液中乙醇浓度为0.01 - 20 g/L的范围内验证了线性关系。测定的灵敏度为0.01 g/L。用该方法测定乙醇简便易行且高度可靠，仅需少量样品体积（0.1 mL）。无需内标和样品处理。所得结果表明，顶空气相色谱法的应用可从确证分析扩展到许多生物医学领域的常规应用。
