采用毛细管柱顶空气相色谱法测定尿液中的酒精。

Headspace gas chromatography with capillary column for urine alcohol determination.

作者信息

Corrêa C L, Pedroso R C

机构信息

Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas da Universidade de São Paulo, Brasil.

出版信息

J Chromatogr B Biomed Sci Appl. 1997 Dec 19;704(1-2):365-8. doi: 10.1016/s0378-4347(97)00445-3.

DOI:10.1016/s0378-4347(97)00445-3

PMID:9518172

Abstract

A headspace gas chromatographic method using a fused-silica capillary column Poraplot Q has been developed and validated for the detection and quantification of ethanol in urine. Under optimized conditions, ethanol was properly separated from acetaldehyde, acetone, isopropanol, methanol and n-propanol. Limits of detection (LODs) and quantification (LOQs) were 0.008 and 0.010 g/l, respectively. The precision studies within-run and between-run, using spiked urine samples (0.08, 0.8 and 2.0 g/l) showed maximum coefficients of variation 5.9 and 6.5%, respectively. Results of ethanol recovery varied from 91.6+/-0.8 to 103.3+/-1.8% over the concentration range from 0.01 to 3.20 g/l. The method was appropriate for the detection of ethanol in urine samples. This matrix can be used for monitoring alcohol abuse in the workplace and used in alcohol rehabilitation programs.

摘要

已开发并验证了一种使用熔融石英毛细管柱Poraplot Q的顶空气相色谱法，用于检测和定量尿液中的乙醇。在优化条件下，乙醇与乙醛、丙酮、异丙醇、甲醇和正丙醇能得到良好分离。检测限（LOD）和定量限（LOQ）分别为0.008和0.010 g/l。使用加标尿液样本（0.08、0.8和2.0 g/l）进行的批内和批间精密度研究显示，最大变异系数分别为5.9%和6.5%。在0.01至3.20 g/l的浓度范围内，乙醇回收率结果在91.6±0.8%至103.3±1.8%之间。该方法适用于检测尿液样本中的乙醇。此基质可用于监测工作场所的酒精滥用情况，并用于酒精康复项目。