Schröder U, Schindler C, Rüsch-Gerdes S, Kövary P M
Forschungsinstitut Borstel.
Pneumologie. 1995 Sep;49(9):502-4.
248 respiratory specimens were examined using by Nested-PCR and by conventional methods (solid and liquid media). 54 specimens were culture-positive for M. tuberculosis, 51 of these were detected by PCR and conventional methods, whereas 3 were missed by PCR and 15 were only positive by PCR. 12 of the 15 culture-negative specimens derived from patients under treatment for tuberculosis. The 3 specimens, which are PCR negative could also be detected after modification of the method. The very high sensitivity depends on the high positive rate (21.8%) of the investigated specimens, so it is necessary to look also for other than pulmonary specimens, but it seems that his Nested-PCR is a highly specific and sensitive method for detecting of M. tuberculosis.
使用巢式聚合酶链反应（Nested-PCR）和传统方法（固体和液体培养基）对248份呼吸道标本进行了检测。54份标本结核分枝杆菌培养呈阳性，其中51份通过PCR和传统方法检测到，而3份PCR未检测到，15份仅PCR呈阳性。15份培养阴性标本中有12份来自正在接受结核病治疗的患者。3份PCR阴性的标本在方法改进后也能被检测到。极高的灵敏度取决于所检测标本的高阳性率（21.8%），因此有必要寻找肺部以外的标本，但似乎这种巢式PCR是一种检测结核分枝杆菌的高度特异性和灵敏的方法。
