Harrison G H, Balcer-Kubiczek E K, Gutierrez P L
Department of Radiation Oncology, University of Maryland School of Medicine, Baltimore 21201, USA.
Radiat Res. 1996 Jan;145(1):98-101.
We compared the ability of continuous-wave ultrasound to enhance cytotoxicity from X irradiation, hyperthermia or exposure to adriamycin. The survival of CHO cells exposed in culture medium to these agents was determined with and without continuous-wave ultrasound (1.62 or 1.765 MHz). In water-filled transmission exposure vessels with 2-cm-diameter Mylar end windows, 10-min insonation not producing cytotoxicity could produce .OH radicals (measured by electron paramagnetic resonance) even at 0.4 W/cm2. Ultrasound at intensities ranging between 1 and 2.5 W/cm2 increased the clonogenic cytotoxicity of adriamycin (P = 0.0023 by paired t test) but not of X rays (2-10 Gy) or hyperthermia (44 degrees C for 10-50 min). The only significant action of continuous-wave ultrasound under similar test conditions was the potentiation of adriamycin-induced clonogenic cytotoxicity, possibly mediated by cavitational activity.
我们比较了连续波超声增强X射线、热疗或阿霉素所致细胞毒性的能力。在有和没有连续波超声（1.62或1.765MHz）的情况下，测定了培养基中暴露于这些药物的CHO细胞的存活率。在具有2厘米直径聚酯薄膜端窗的充水传输暴露容器中，即使在0.4W/cm²的强度下，10分钟的超声照射（未产生细胞毒性）也能产生·OH自由基（通过电子顺磁共振测量）。强度在1至2.5W/cm²之间的超声增加了阿霉素的克隆形成细胞毒性（配对t检验，P = 0.0023），但对X射线（2 - 10Gy）或热疗（44℃，持续10 - 50分钟）没有影响。在类似测试条件下，连续波超声的唯一显著作用是增强阿霉素诱导的克隆形成细胞毒性，可能由空化活性介导。
