Heinlein M, Epel B L, Padgett H S, Beachy R N
Scripps Research Institute, Department of Cell Biology, La Jolla, CA 92037, USA.
Science. 1995 Dec 22;270(5244):1983-5. doi: 10.1126/science.270.5244.1983.
The movement protein of tobacco mosaic tobamovirus and related viruses is essential for the cell-to-cell spread of infection and, in part, determines the host range of the virus. Movement protein (MP) was fused with the jellyfish green fluorescent protein (GFP), and a modified virus that contained this MP:GFP fusion protein retained infectivity. In protoplasts and leaf tissues, the MP:GFP fusion protein was detected as long filaments shortly after infection. Double-labeling fluorescence microscopy suggests that the MP interacts and coaligns with microtubules. The distribution of the MP is disrupted by treatments that disrupt microtubules, but not by cytochalasin B, which disrupts filamentous F-actin. Microtubules may target the MP to plasmodesmata, the intercellular channels that connect adjacent cells.
烟草花叶烟草花叶病毒及相关病毒的运动蛋白对于感染的细胞间传播至关重要，并且在一定程度上决定了病毒的宿主范围。运动蛋白（MP）与水母绿色荧光蛋白（GFP）融合，含有这种MP:GFP融合蛋白的修饰病毒保留了感染性。在原生质体和叶片组织中，感染后不久MP:GFP融合蛋白就被检测为长丝。双标记荧光显微镜表明MP与微管相互作用并共排列。MP的分布会被破坏微管的处理所扰乱，但不会被破坏丝状F-肌动蛋白的细胞松弛素B所扰乱。微管可能将MP靶向到胞间连丝，即连接相邻细胞的细胞间通道。
