Kraupp M, Marz R
Institut für Medizinische Chemie, Universität Wien.
Wien Klin Wochenschr. 1995;107(22):677-80.
Membrane transport of nucleobases and nucleosides has been an actively pursued research field for the past 25 years. Not only are these substances of physiological interest; derivatives are in clinical use or under investigation for their pharmacological activity against viral and neoplastic disease. An understanding of the molecular pharmacology of these substances includes a detailed knowledge of how they reach their intracellular targets. Membrane transport systems which have so far been found in all cells examined play an important role in this process. Since the transporters are minor membrane components, little is known about them on a molecular basis. This review discusses methodological approaches used to measure initial rates of membrane transport and summarizes current knowledge of the various transport systems which have been characterized with these kinetic methods.
在过去25年里，核碱基和核苷的膜转运一直是一个积极探索的研究领域。这些物质不仅具有生理意义；其衍生物已在临床使用，或因其对病毒和肿瘤疾病的药理活性而正在接受研究。对这些物质分子药理学的理解包括对它们如何到达细胞内靶点的详细了解。迄今为止，在所有被检测的细胞中发现的膜转运系统在这一过程中起着重要作用。由于转运蛋白是膜的次要成分，在分子层面上对它们的了解很少。本综述讨论了用于测量膜转运初始速率的方法，并总结了用这些动力学方法已表征的各种转运系统的现有知识。
