Vidziunaité R, Mikulskis P, Kulys J
Institute of Biochemistry, Lithuania.
J Biolumin Chemilumin. 1995 Jul-Aug;10(4):199-203. doi: 10.1002/bio.1170100402.
The detection of brucellosis and tularaemia infection agents is of particular interest for medical practice. The possibility of using enhanced chemiluminescence reactions for the determination of these agents is studied in this work. Light intensity depends on both the conjugate concentration used and the conditions at which the adsorption was performed. Optimal conditions for these test-systems were: approximately 20 micrograms/mL of Ig and 200 micrograms/mL (titre 1:20) of conjugate. As is seen from the chemiluminescent and spectrophotometric results the lowest determined concentrations are 10 and 30 ng/mL (for brucellosis) and 1 and 5 ng/mL (for tularaemia), respectively. Calibration curves in the antigen concentrations ranging from 10 to 2500 ng/mL (for brucellosis) and from 1 to 500 ng/mL (for tularaemia) are observed. Optical density depends linearly on the logarithm of the antigen concentration from 30 to 5000 ng/mL (for brucellosis) and from 5 to 250 ng/mL (for tularaemia). The results obtained permit the conclusion that the chemiluminescence method can be used in enzyme immunoanalysis for brucellosis and tularaemia antigens.
布鲁氏菌病和兔热病感染因子的检测对医学实践尤为重要。本研究探讨了利用增强化学发光反应来测定这些感染因子的可能性。光强度既取决于所用共轭物的浓度，也取决于进行吸附的条件。这些检测系统的最佳条件为：约20微克/毫升的免疫球蛋白和200微克/毫升（效价1:20）的共轭物。从化学发光和分光光度法结果可以看出，最低测定浓度分别为10和30纳克/毫升（针对布鲁氏菌病）以及1和5纳克/毫升（针对兔热病）。观察到抗原浓度范围为10至2500纳克/毫升（针对布鲁氏菌病）和1至500纳克/毫升（针对兔热病）的校准曲线。光密度在抗原浓度为30至5000纳克/毫升（针对布鲁氏菌病）和5至250纳克/毫升（针对兔热病）时与抗原浓度的对数呈线性关系。所得结果表明，化学发光法可用于布鲁氏菌病和兔热病抗原的酶免疫分析。
