Ueda M, Tanabe J, Inomata M, Kaneko A, Kimura T
Department of Ophthalmology, Kanazawa University School of Medicine, Ishikawa-ken, Japan.
Nippon Ganka Gakkai Zasshi. 1995 Nov;99(11):1230-5.
Effects of an intravitreal injection of melphalan on the electroretinogram and on the retinal structure were studied in albino rabbits to establish the non-toxic dose for its intravitreal use. The a-wave, the b-wave, the c-wave, the oscillatory potential and the retinal structure remained unchanged after 10-micrograms injection, but moderately changed after 20-micrograms injection and greatly deteriorated after 90-micrograms injection. A 10-micrograms injection is equal to an intravitreal concentration of about 5.9 micrograms/ml, if evenly diffused in the rabbit vitreous. Considering that colony formation of in vitro retinoblastoma cells is completely suppressed by melphalan at 4 micrograms/ml concentration, an intravitreal application of melphalan could be used as a non-surgical treatment for retinoblastoma.
为确定美法仑玻璃体内使用的无毒剂量，对白化兔进行了玻璃体内注射美法仑对视网膜电图和视网膜结构影响的研究。注射10微克后，a波、b波、c波、振荡电位和视网膜结构保持不变，但注射20微克后有中度变化，注射90微克后则严重恶化。如果在兔玻璃体内均匀扩散，10微克的注射量相当于玻璃体内浓度约为5.9微克/毫升。鉴于在4微克/毫升浓度下美法仑可完全抑制体外视网膜母细胞瘤细胞的集落形成，玻璃体内应用美法仑可作为视网膜母细胞瘤的非手术治疗方法。
