Watson G L, Slocombe R F, Robinson N E, Sleight S D
Department of Pathology, Michigan State University, East Lansing 48824, USA.
Am J Vet Res. 1995 Aug;56(8):1055-61.
Release of enzymes from cytoplasmic granules has been postulated to have a major role in neutrophil-mediated tissue injury. Secretion or release of primary granules, specific granules, and cytosolic enzymes by bovine neutrophils was examined by quantifying the release of beta-glucuronidase, B12-binding protein, and lactate dehydrogenase, respectively, in response to predetermined amounts of phorbol myristate acetate, calcium ionophore, and opsonized zymosan. These responses were compared with the enzyme release induced by exposure to live or dead, unopsonized or opsonized Pasteurella haemolytica. The greatest release of beta-glucuronidase, B12-binding protein, and lactate dehydrogenase was observed in neutrophils exposed to live organisms partially because of neutrophil lysis. Bovine neutrophils respond markedly to particulate agonists, live or dead, pathogenic or nonpathogenic, by a selective release of specific granules, an effect enhanced by opsonization. Particulate agonists induce minimal primary granule release other than that induced by cell death. Because bovine neutrophils contain quantitatively high numbers of specific granules, the high rate of secretion/release in response to P haemolytica organisms could have a major role in the tissue responses that characterize the lesions of pneumonic pasteurellosis.
细胞质颗粒中酶的释放被认为在中性粒细胞介导的组织损伤中起主要作用。通过分别定量β-葡萄糖醛酸酶、维生素B12结合蛋白和乳酸脱氢酶的释放，检测了牛中性粒细胞中初级颗粒、特异性颗粒和胞质酶的分泌或释放情况，这些释放是对预先确定量的佛波酯肉豆蔻酸酯、钙离子载体和调理酵母聚糖的反应。将这些反应与暴露于活的或死的、未调理或调理的溶血巴斯德菌诱导的酶释放进行比较。在暴露于活生物体的中性粒细胞中观察到β-葡萄糖醛酸酶、维生素B12结合蛋白和乳酸脱氢酶的最大释放，部分原因是中性粒细胞溶解。牛中性粒细胞对颗粒性激动剂（活的或死的、致病的或非致病的）有明显反应，通过选择性释放特异性颗粒，这种作用因调理作用而增强。颗粒性激动剂除了诱导细胞死亡外，诱导的初级颗粒释放极少。由于牛中性粒细胞含有数量较多的特异性颗粒，对溶血巴斯德菌生物体的高分泌/释放率可能在表征肺炎巴氏杆菌病病变的组织反应中起主要作用。
