Salt A N, DeMott J E, Kimura R S
Department of Otolaryngology, Washington University Medical School, St Louis, Missouri 63110, USA.
Ann Otol Rhinol Laryngol. 1995 Nov;104(11):886-94. doi: 10.1177/000348949510401111.
In order to establish how endolymph volume is regulated, it is essential to be able to measure endolymph volume or cross-sectional area in vivo. We have developed methods to accomplish this by injecting the volume marker ion hexafluoroarsenate (AsF6) into endolymph by iontophoresis. For an injection at a constant rate, the endolymph concentration is inversely dependent on the cross-sectional area of the scala into which injection occurred. Marker concentrations were monitored by inserting ion-selective microelectrodes into endolymph near the injection site. In a previous study we quantified the degree of hydrops in animals following ablation of the endolymphatic sac. In the present study we validated the technique by comparing the endolymphatic cross-sectional area measured in vivo with AsF6 with that measured by established histologic procedures. The correlation between the two measures was good, with a coefficient of .903, although the area measured histologically was a little lower than that measured in vivo.
为了确定内淋巴液体积是如何调节的，能够在体内测量内淋巴液体积或横截面积至关重要。我们已开发出通过离子电渗法将体积标记物六氟砷酸盐离子（AsF6）注入内淋巴来实现这一目的的方法。以恒定速率进行注射时，内淋巴浓度与注射所进入的蜗管横截面积成反比。通过将离子选择性微电极插入注射部位附近的内淋巴中来监测标记物浓度。在之前的一项研究中，我们对动物内淋巴囊切除术后的积水程度进行了量化。在本研究中，我们通过比较用AsF6在体内测量的内淋巴横截面积与用既定组织学程序测量的结果，对该技术进行了验证。尽管组织学测量的面积略低于体内测量值，但两种测量方法之间的相关性良好，系数为0.903。
