低氧性缺氧在大鼠脑左右半球皮质中引发不同的生化变化。

Hypoxic hypoxia induces different biochemical changes in the cortex of the right and left hemispheres of rat brain.

作者信息

Nalivaeva N, Plesneva A, Chekulaeva U, Zhuravin I, Dubrovskaya N, Klementjev B

机构信息

I. M. Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Science, St. Petersburg, Russia.

出版信息

Mol Chem Neuropathol. 1995 Jun-Aug;25(2-3):255-63. doi: 10.1007/BF02960918.

DOI:10.1007/BF02960918

PMID:8534326

Abstract

The activity of acetylcholinesterase (AChE), adenylate cyclase (AC), 5'-nucleotidase (NT), Na+, K(+)-ATPase, as well as the contents of phospholipids (PL) and gangliosides (G) per mg of protein in homogenate, crude membrane (P2) fraction, and synaptosomes from the sensorimotor cortex of the right and left hemispheres of rat brain were analyzed under normal and hypoxic conditions. The authors found that under normal physiological conditions there are no significant differences of the studied parameters in homogenates of sensorimotor cortex from the right and left hemispheres. In P2 fractions, and especially in preparations of synaptosomes from the right and left cortex, differences in the activity of 5'-NT and AC were found. Hypoxia (pO2 = 7.8%) was shown to alter studied parameters (AChE, AC, Na+, K(+)-ATPase activity, and PL content) mainly in the right hemisphere.

摘要

在正常和缺氧条件下，分析了大鼠脑左右半球感觉运动皮层匀浆、粗膜（P2）组分和突触体中每毫克蛋白质的乙酰胆碱酯酶（AChE）、腺苷酸环化酶（AC）、5'-核苷酸酶（NT）、Na +、K（+）-ATP酶的活性，以及磷脂（PL）和神经节苷脂（G）的含量。作者发现，在正常生理条件下，左右半球感觉运动皮层匀浆中所研究的参数没有显著差异。在P2组分中，尤其是在左右皮层的突触体制备中，发现了5'-NT和AC活性的差异。结果表明，缺氧（pO2 = 7.8%）主要改变右半球的研究参数（AChE、AC、Na +、K（+）-ATP酶活性和PL含量）。