Piaton E, Bringuier P P, Seigneurin D, Perrin P, Devonec M
Laboratoire d'Histologie, Faculté de Médecine Lyon Grange-Blanche, France.
Anal Quant Cytol Histol. 1995 Oct;17(5):351-60.
To compare the DNA content measured by flow cytometry (FCM) and image analysis (IA) from prostatic fine needle aspiration (FNA) samples.
A total of 48 samples were studied. FCM was performed on propidium iodide-stained nuclei according to the Vindelov method, and image analysis was performed on Feulgen-stained slides. Positive FNA results were grade (1-3) and compared with Gleason grades.
Aneuploidy was closely related to positive FNA results (P < .01). DNA histograms were found to be aneuploid in all grade 3 carcinomas (n = 13) by IA and in 11 cases (84.6%) by FCM. Grade 2 carcinomas (n = 9) were found to be aneuploid with both methods. In grade 1 carcinomas (n = 10), 2 cases exhibited IA aneuploid profiles, whereas all FCM cases were diploid. Aneuploid profiles were more often associated with high Gleason scores than were diploid ones (P < .01). Among the 16 patients with negative FNA results, two cases had tetraploid DNA profiles related to contaminating seminal vesicle cells. The difference in DNA measurements reached 10.4% but was not statistically significant.
These findings show that the two methods, as applied to prostatic FNA samples, give comparable results and that seminal vesicle cells may be responsible for false tetraploid profiles.
比较流式细胞术（FCM）和图像分析（IA）测量前列腺细针穿刺（FNA）样本的DNA含量。
共研究了48个样本。根据温德洛夫方法对碘化丙啶染色的细胞核进行FCM检测，对福尔根染色的玻片进行图像分析。FNA阳性结果分为1-3级，并与 Gleason分级进行比较。
非整倍体与FNA阳性结果密切相关（P <.01）。通过IA发现所有3级癌（n = 13）的DNA直方图为非整倍体，通过FCM发现11例（84.6%）为非整倍体。两种方法均发现2级癌（n = 9）为非整倍体。在1级癌（n = 10）中，2例呈现IA非整倍体图谱，而所有FCM检测病例均为二倍体。与二倍体图谱相比，非整倍体图谱更常与高Gleason评分相关（P <.01）。在16例FNA结果为阴性的患者中，2例有与精囊细胞污染相关的四倍体DNA图谱。DNA测量的差异达到10.4%，但无统计学意义。
这些发现表明，应用于前列腺FNA样本的这两种方法结果相当，精囊细胞可能是导致假四倍体图谱的原因。
