Horák V
Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Libĕchov, Czech Republic.
Reprod Nutr Dev. 1995;35(6):725-36. doi: 10.1051/rnd:19950612.
Histochemical differentiation of 12 skeletal muscles with a different fibre type composition was studied in miniature pigs from 80 d of gestation to 1 year of age. Two fetal myofibre types were distinguished at 100 d of gestation by the mATPase reaction after acid preincubation. The staining for oxidative enzyme activities showed no conspicuous differences between fibres up to the 6th day after birth. Starting from this age it was possible to distinguish 3 fibre categories: SO (slow-twitch oxidative); FOG (fast-twitch oxidative-glycolytic); and FG (fast-twitch glycolytic). A characteristic cluster distribution of the 3 fibre types was observed in all studied muscles with the exception of the masseter muscle which consisted only of the type SO and FOG fibres with a mosaic arrangement. The frequencies of both SO and FG fibre types increased and the proportion of type FOG fibres decreased during the postnatal period. These changes could be explained by developmental transformations among the individual fibre types. The type FOG fibres converted preferably to the fibre type (SO or FG) that prevailed in the muscles of adult animals.
在从妊娠80天到1岁的小型猪中，研究了12种具有不同纤维类型组成的骨骼肌的组织化学分化。在妊娠100天时，通过酸预孵育后的mATPase反应区分出两种胎儿肌纤维类型。出生后第6天之前，纤维之间氧化酶活性的染色没有明显差异。从这个年龄开始，可以区分出3种纤维类别：SO（慢肌纤维氧化型）；FOG（快肌纤维氧化-糖酵解型）；和FG（快肌纤维糖酵解型）。除咬肌仅由SO型和FOG型纤维呈镶嵌排列组成外，在所有研究的肌肉中均观察到3种纤维类型的特征性簇状分布。在出生后时期，SO型和FG型纤维的频率增加，而FOG型纤维的比例下降。这些变化可以通过个体纤维类型之间的发育转变来解释。FOG型纤维优先转变为成年动物肌肉中占优势的纤维类型（SO或FG）。
