Ozaki T, Irie K, Sakiyama S
Division of Biochemistry, Chiba Cancer Center Research Institute, Japan.
DNA Cell Biol. 1995 Dec;14(12):1017-23. doi: 10.1089/dna.1995.14.1017.
A cDNA clone, termed N17, was isolated from a rat fibroblast 3Y1 cDNA library by a differential screening technique. The expression of the N17 gene was significantly increased in a variety of rapidly growing cells, including v-src-, v-Ha-ras-, or v-mos-transformed 3Y1 cells, when compared with parental 3Y1 cells. The N17 gene is present as a single copy in rat genome and is evolutionarily conserved among higher eukaryotes. The predicted open reading frame (ORF) encodes a polypeptide of 379 amino acids that exhibits a significant similarity with those of the cell cycle control protein Cdc37. The amount of N17 mRNA starts to be increased in the late G1 phase and the same level was retained until just before the G2/M phase. Taken together, these results suggest that N17 gene product may play a crucial role in the cell cycle control of 3Y1 cells.
通过差异筛选技术，从大鼠成纤维细胞3Y1 cDNA文库中分离出一个名为N17的cDNA克隆。与亲代3Y1细胞相比，N17基因在多种快速生长的细胞中表达显著增加，包括v-src、v-Ha-ras或v-mos转化的3Y1细胞。N17基因在大鼠基因组中以单拷贝形式存在，并且在高等真核生物中具有进化保守性。预测的开放阅读框（ORF）编码一个379个氨基酸的多肽，该多肽与细胞周期控制蛋白Cdc37具有显著相似性。N17 mRNA的量在G1期晚期开始增加，并在G2/M期之前一直保持在相同水平。综上所述，这些结果表明N17基因产物可能在3Y1细胞的细胞周期控制中起关键作用。
