Manalaysay M T, Kumano K, Hyodo T, Sakai T
Kidney Center, Kitasato University Hospital, Kanagawa, Japan.
Adv Perit Dial. 1995;11:239-42.
Heparin, routinely used in peritoneal dialysis, has been found to have various effects, mainly inhibitory. Its infusion into the peritoneal cavity may adversely affect mesothelial cell growth and regeneration. Thus we wished to determine if heparin inhibits mesothelial cell growth and is does-dependent. Rat mesothelial cells were incubated for 3 days and 6 days with 200, 100, 20, 2, and 0.5 U/mL of heparin. Cellular proliferation and protein synthesis were determined by liquid scintillation counting after 24-hour pulses with 3H-thymidine and 3H-leucine. Heparin at 2 and 0.5 U/mL had no significant effect on radionuclide uptake. At 100 U/mL heparin inhibited radionuclide uptake by at least 50%. These findings indicate that heparin in small concentrations may be harmless, but at higher concentrations is inhibitory to mesothelial cell growth and proliferation.
肝素常用于腹膜透析，已发现它有多种作用，主要是抑制作用。将其注入腹腔可能会对间皮细胞的生长和再生产生不利影响。因此，我们希望确定肝素是否抑制间皮细胞生长以及是否存在剂量依赖性。用200、100、20、2和0.5 U/mL的肝素将大鼠间皮细胞孵育3天和6天。在用³H-胸腺嘧啶核苷和³H-亮氨酸进行24小时脉冲标记后，通过液体闪烁计数法测定细胞增殖和蛋白质合成。2和0.5 U/mL的肝素对放射性核素摄取没有显著影响。100 U/mL的肝素至少抑制50%的放射性核素摄取。这些发现表明，低浓度的肝素可能无害，但高浓度时会抑制间皮细胞的生长和增殖。
