豌豆质体蓝素基因翻译起始密码子周围的序列提高了报告基因的翻译效率。
The sequence surrounding the translation initiation codon of the pea plastocyanin gene increases translational efficiency of a reporter gene.
作者信息
Helliwell C A, Gray J C
机构信息
Department of Plant Sciences, University of Cambridge, UK.
出版信息
Plant Mol Biol. 1995 Nov;29(3):621-6. doi: 10.1007/BF00020990.
The 5'-upstream region of the pea plastocyanin gene (petE) directed 5-10-fold higher levels of beta-glucuronidase (GUS) activity than the cauliflower mosaic virus 35S promoter in transgenic tobacco plants, although the levels of GUS mRNA were similar. The sequence (AAAAAUGG) around the translation initiation codon of petE enhanced translation of the GUS mRNA 10-fold compared to translation from the GUS translation initiation codon in transgenic tobacco plants and transfected protoplasts.
豌豆质体蓝素基因（petE）的5'上游区域在转基因烟草植株中指导的β-葡萄糖醛酸酶（GUS）活性水平比花椰菜花叶病毒35S启动子高5至10倍，尽管GUS mRNA的水平相似。与转基因烟草植株和转染原生质体中从GUS翻译起始密码子开始的翻译相比，petE翻译起始密码子周围的序列（AAAAAUGG）使GUS mRNA的翻译增强了10倍。
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