Asano T, McIntyre B W, Bednarczyk J L, Wygant J N, Kleinerman E S
Department of Cell Biology, University of Texas M.D. Anderson Cancer Center, Houston 77030, USA.
Oncol Res. 1995;7(5):253-7.
We previously demonstrated that liposome-encapsulated muramyl tripeptide phosphatidylethanolamine (L-MTP-PE), a biologic response modifier now undergoing phase III clinical trial in osteosarcoma, upregulated monocyte expression of several cytokines' mRNA and the subsequent production of these proteins. In the present work, we investigated whether L-MTP-PE upregulated adhesion molecules on the surface of normal human monocytes. Flow-cytometric analysis showed that several subunits of the integrins, including alpha L, alpha 5, and beta 1 subunits, and intercellular adhesion molecule-1 on the monocytes were upregulated following their stimulation with 2 micrograms/ml L-MTP-PE for 24 h. Anti-alpha L antibodies blocked monocyte-mediated tumor cell killing stimulated by L-MTP-PE. We conclude that L-MTP-PE also stimulates the increase of several molecules on the monocyte cell surface. These adhesion molecules may contribute to the increased activation of monocyte-mediated tumor cell killing seen following L-MTP-PE exposure.
我们之前证明，脂质体包裹的胞壁酰三肽磷脂酰乙醇胺（L-MTP-PE），一种目前正在骨肉瘤中进行III期临床试验的生物反应调节剂，上调了几种细胞因子mRNA在单核细胞中的表达以及这些蛋白质的后续产生。在本研究中，我们调查了L-MTP-PE是否上调正常人单核细胞表面的黏附分子。流式细胞术分析表明，在用2微克/毫升L-MTP-PE刺激单核细胞24小时后，整合素的几个亚基，包括αL、α5和β1亚基，以及细胞间黏附分子-1均上调。抗αL抗体阻断了L-MTP-PE刺激的单核细胞介导的肿瘤细胞杀伤。我们得出结论，L-MTP-PE也刺激单核细胞表面几种分子的增加。这些黏附分子可能有助于L-MTP-PE暴露后单核细胞介导的肿瘤细胞杀伤激活增加。
