Liechty M C, Hall B K, Scalzi J M, Davis L M, Caspary W J, Hozier J C
Applied Genetics Laboratories, Inc, Melbourne, Florida 32901, USA.
Mamm Genome. 1995 Sep;6(9):592-4. doi: 10.1007/BF00352363.
Using degenerate primer amplification of chromosomes microdissected from banded cytogenetic preparations, we constructed both whole chromosome painting probes for mouse Chromosomes (Chrs) 1, 2, 3, and 11 and a centromere probe that strongly paints most mouse centromeres. We also amplified a Robertsonian translocation chromosome microdissected from unstained preparations to construct a painting probe for Chrs 9 and 19. The chromosome probes uniformly painted the respective chromosomes of origin. We demonstrated the utility of the Chr 11 probe in aberration analysis by staining mutants that we had previously identified as containing a Chr 11 translocation, and in some mutant cell lines we observed chromosome rearrangements not previously detected in stained cytogenetic preparations. The technology of microdissection and amplification applies to all mouse chromosomes or to specific subchromosomal regions and will be useful in mouse genetics, in aberration analysis, and for chromosome identification.
利用从显带细胞遗传学标本中显微切割的染色体进行简并引物扩增，我们构建了用于小鼠1号、2号、3号和11号染色体的全染色体涂染探针以及一个能强烈涂染大多数小鼠着丝粒的着丝粒探针。我们还从未染色的标本中显微切割出一条罗伯逊易位染色体进行扩增，构建了用于9号和19号染色体的涂染探针。这些染色体探针能均匀地涂染各自的起源染色体。我们通过对先前鉴定为含有11号染色体易位的突变体进行染色，证明了11号染色体探针在畸变分析中的实用性，并且在一些突变细胞系中我们观察到了在染色的细胞遗传学标本中先前未检测到的染色体重排。显微切割和扩增技术适用于所有小鼠染色体或特定的亚染色体区域，将在小鼠遗传学、畸变分析和染色体鉴定中发挥作用。
