Skutella T, Probst J C, Jirikowski G F
Max-Planck Institute of Psychiatry, Dept. of Neuroendocrinology, München, Germany.
Cell Mol Biol (Noisy-le-grand). 1995 Sep;41(6):793-8.
In situ hybridization revealed that c-fos encoding transcripts occur in the median eminence of rats. Osmotic stress resulted within 15 min. in additional labeling of the magnocellular nuclei, while osmotically stimulated rats that had been pretreated with colchicine failed to show c-fos hybridization in the magnocellular perikarya. Rats, pretreated with a polymerase II inhibitor, showed 15 min. after osmotic stimulation c-fos hybridization in the hypothalamic nuclei, whereas the median eminence was depleted of hybridization signal. c-fos is probably among the transcripts, stored in axons of the hypothalamo-neurohypophysial system, to be transported retrogradly upon osmotic stimulation.
原位杂交显示，编码c-fos的转录本出现在大鼠的正中隆起。渗透压应激在15分钟内导致大细胞神经核有额外标记，而预先用秋水仙碱处理的经渗透压刺激的大鼠，其大细胞神经元胞体未显示c-fos杂交信号。预先用聚合酶II抑制剂处理的大鼠，在渗透压刺激15分钟后，下丘脑神经核出现c-fos杂交信号，而正中隆起的杂交信号消失。c-fos可能是储存在下丘脑-神经垂体系统轴突中的转录本之一，在渗透压刺激时逆行运输。
