Bermúdez Moretti M, Correa García S, Ramos E H, Batlle A
Centro de Investigaciones sobre Porfirinas y Porfirias, CIPYP (CONICET, FCEyN, UBA), Ciudad Universitaria, Buenos Aires, Argentina.
Cell Mol Biol (Noisy-le-grand). 1995 Sep;41(6):843-9.
The aim of this work was to characterize the 4-aminobutyric acid (GABA) transport in the Saccharomyces cerevisiae D27 strain, followed by the study of the relationship between 5-aminolevulinic acid (ALA) and GABA transport systems. It was found that the general amino acid permease (GAP) is not active in D27 strain, suggesting that GABA incorporation should be mediated by PUT4 and UGA4 permeases. However, after kinetic studies only one system was detected. It was also shown that GABA uptake is competitively inhibited by ALA. GABA incorporation is regulated by the carbon source but not by the nitrogen source. When cells were grown in the presence of GABA, its entrance was very low.
这项工作的目的是表征酿酒酵母D27菌株中的4-氨基丁酸（GABA）转运，随后研究5-氨基乙酰丙酸（ALA）与GABA转运系统之间的关系。研究发现，普通氨基酸通透酶（GAP）在D27菌株中无活性，这表明GABA的掺入应由PUT4和UGA4通透酶介导。然而，经过动力学研究，仅检测到一个系统。研究还表明，ALA对GABA的摄取具有竞争性抑制作用。GABA的掺入受碳源调节，但不受氮源调节。当细胞在GABA存在下生长时，其进入量非常低。
