Weiss L, Reich S, Slavin S
Department of Bone Marrow Transplantation, Hadassah University Hospital, Jerusalem, Israel.
Bone Marrow Transplant. 1995 Sep;16(3):457-61.
The role of various cell subpopulations involved in the graft-versus-leukemia (GVL) effect induced by allogeneic bone marrow transplantation (BMT) was investigated in (BALB/c x C57BL/6)F1 (F1) recipients, inoculated with murine B cell leukemia (BCL1), by using monoclonal murine anti-IL-2 receptor antibodies or rabbit anti-Asialo GM1 antibodies directed predominantly against murine NK cells. F1 mice with BCL1 were irradiated and reconstituted with parental (C57BL/6) bone marrow cells (BMC) or a mixture of BMC and spleen cells and treated in vivo for 10 days with anti-IL-2 receptor antibody or anti-Asialo-GM1 antibody. Both treatments lessened the GVL effects induced by the allograft and resulted in development of leukemia relapse, as documented in vivo by adoptive transfer of 10(5) spleen cells obtained from treated mice into secondary syngeneic adoptive recipients. Our data indicate that IL-2 receptor-positive T cells and possibly IL-2-activated allogeneic NK cells play a key role in inducing GVL following allogeneic BMT.
通过使用主要针对小鼠NK细胞的单克隆小鼠抗IL-2受体抗体或兔抗去唾液酸GM1抗体，在接种了小鼠B细胞白血病（BCL1）的（BALB/c×C57BL/6）F1（F1）受体小鼠中，研究了参与同种异体骨髓移植（BMT）诱导的移植物抗白血病（GVL）效应的各种细胞亚群的作用。将患有BCL1的F1小鼠进行照射，并用亲代（C57BL/6）骨髓细胞（BMC）或BMC与脾细胞的混合物进行重建，并用抗IL-2受体抗体或抗去唾液酸GM1抗体在体内处理10天。两种处理均减轻了同种异体移植诱导的GVL效应，并导致白血病复发，这通过将从处理过的小鼠获得的10⁵个脾细胞过继转移到二级同基因过继受体小鼠体内而在体内得到证实。我们的数据表明，IL-2受体阳性T细胞以及可能被IL-2激活的同种异体NK细胞在同种异体BMT后诱导GVL中起关键作用。
