Nielsen A H, Hagemann A, Avery B, Poulsen K
Department of Anatomy and Physiology, Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
Exp Clin Endocrinol Diabetes. 1995;103(5):332-8. doi: 10.1055/s-0029-1211374.
The expression of angiotensin (Ang) II receptors, active renin and prorenin in porcine and bovine ovarian follicles and corpora lutea was investigated and compared. In the wall tissue of porcine follicles, the Ang II receptor density was 47 (range 19-97; n = 13) fmol/mg membrane protein. The active renin concentration was 1.32 (0.40-3.43; n = 23) GU/kg wet tissue. These values were about 35-fold and 15-fold lower, respectively, than previously found in bovine follicles. No prorenin could be detected in the porcine follicular wall tissue. Ang II receptors of subtype 2 (AT2 receptors) with a dissociation constant (Kd) of 1.01 (0.64-1.79; n = 8) nmol/l for [Sar1-Ile5-Ile8]-Ang II were demonstrated in the bovine corpus luteum. The receptor density was 22.7 (1.9-93; n = 26) fmol/mg membrane protein, which was about 10-fold higher than in porcine corpora lutea. The active renin concentration was 20.7 (2.2-60.0; n = 26) GU/kg tissue in bovine and 0.40 (0.16-1.09; n = 17) GU/kg tissue in porcine corpora lutea. No prorenin could be detected in corpora lutea from both species. The variation between species in expression of the ovarian renin-angiotensin system indicates the existence of species differences in the physiological role.
研究并比较了血管紧张素（Ang）II受体、活性肾素和肾素原在猪和牛卵巢卵泡及黄体中的表达。在猪卵泡壁组织中，Ang II受体密度为47（范围19 - 97；n = 13）fmol/mg膜蛋白。活性肾素浓度为1.32（0.40 - 3.43；n = 23）GU/kg湿组织。这些值分别比先前在牛卵泡中发现的值低约35倍和15倍。在猪卵泡壁组织中未检测到肾素原。在牛黄体中证实了对[Sar1 - Ile5 - Ile8]-Ang II的解离常数（Kd）为1.01（0.64 - 1.79；n = 8）nmol/l的2型Ang II受体（AT2受体）。受体密度为22.7（1.9 - 93；n = 26）fmol/mg膜蛋白，比猪黄体中的约高10倍。牛黄体中的活性肾素浓度为20.7（2.2 - 60.0；n = 26）GU/kg组织，猪黄体中的为0.40（0.16 - 1.09；n = 17）GU/kg组织。在两个物种的黄体中均未检测到肾素原。卵巢肾素 - 血管紧张素系统表达的种间差异表明在生理作用方面存在种间差异。
