绵羊线粒体DNA D环及其侧翼tRNA基因的核苷酸序列。

Nucleotide sequence of the sheep mitochondrial DNA D-loop and its flanking tRNA genes.

作者信息

Zardoya R, Villalta M, López-Pérez M J, Garrido-Pertierra A, Montoya J, Bautista J M

机构信息

Departamento de Bioquímica y Biología Molecular IV, Universidad Complutense de Madrid, Facultad de Veterinaria, Spain.

出版信息

Curr Genet. 1995 Jun;28(1):94-6. doi: 10.1007/BF00311887.

DOI:10.1007/BF00311887

PMID:8536319

Abstract

The nucleotide sequence of the sheep mitochondrial DNA displacement-loop (D-loop) region and its flanking tRNA genes has been determined. Several conserved motifs among mammals have been identified along the 1189-bp sequence of the sheep control region: ten termination-associated sequences (TASs) and one conserved sequence block (CSB-1). CSB-2 and CSB-3, which are frequently found in most species, are not present in the sheep D-loop, which shows instead a short direct repeat at their usual localization. A long polypyrimidine tract between CSB-1 and the tRNA(Phe) gene is also present. Furthermore, the sheep mtDNA D-loop region displays tandem repeats in the left domain (adjacent to the tRNA(Pro) gene) comprising three different termination-associated sequences (TAS-5, TAS-6 and TAS-7).

摘要

已确定绵羊线粒体DNA置换环（D环）区域及其侧翼tRNA基因的核苷酸序列。沿着绵羊控制区1189bp的序列，已鉴定出哺乳动物中几个保守基序：十个终止相关序列（TAS）和一个保守序列块（CSB-1）。在大多数物种中经常发现的CSB-2和CSB-3在绵羊D环中不存在，相反，在其通常定位处显示出短的直接重复序列。在CSB-1和tRNA（Phe）基因之间也存在一个长的多嘧啶序列。此外，绵羊线粒体DNA D环区域在左结构域（与tRNA（Pro）基因相邻）显示串联重复序列，其包含三个不同的终止相关序列（TAS-5、TAS-6和TAS-7）。