Giannarelli S, Nano J L, Fournel S, Rampal P
Laboratoire de Gastroentérologie et de Nutrition, UFR de Médecine, Nice, France.
Digestion. 1995;56(6):509-15. doi: 10.1159/000201284.
In addition to their inhibitory action on gastric acid secretion, prostaglandins may exert part of their protective effect on the gastrointestinal mucosa by specifically maintaining the cellular integrity of the intestinal epithelium. This in vitro study investigated the cytoprotective effect conferred on intestinal epithelial cell lines IRD 98 and IEC 17 against ethanol injury by the synthetic prostaglandin enprostil and compared effects with those of the histamine H2-receptor blocker cimetidine. Exposure to 3% ethanol (652 mM) reduced cell viability and increased the cAMP and membrane fluidity of both cell lines. Our results demonstrate that: (i) enprostil exerts a significant cytoprotective effect against damage by ethanol; (ii) cimetidine has no cytoprotective effect; (iii) IRD 98 cells are more sensitive to enprostil than IEC 17 cells.
除了对胃酸分泌有抑制作用外，前列腺素还可能通过特异性维持肠上皮细胞的完整性，对胃肠道黏膜发挥部分保护作用。这项体外研究调查了合成前列腺素恩前列素对肠上皮细胞系IRD 98和IEC 17免受乙醇损伤的细胞保护作用，并将其与组胺H2受体阻滞剂西咪替丁的作用进行了比较。暴露于3%乙醇（652 mM）会降低两种细胞系的细胞活力，并增加其环磷酸腺苷（cAMP）和膜流动性。我们的结果表明：（i）恩前列素对乙醇损伤具有显著的细胞保护作用；（ii）西咪替丁没有细胞保护作用；（iii）IRD 98细胞比IEC 17细胞对恩前列素更敏感。
