Flores-Romo L, Heneine L G, Heneine I F
Department of Immunology, Birmingham University, England.
Immunopharmacol Immunotoxicol. 1995 Nov;17(4):783-9. doi: 10.3109/08923979509037196.
The venom from the snake Crotalus durissus terrificus was detoxified by stepwise incorporation of stable cationic iodine. The venoid, in a dosage equivalent to 100 LD50 of the lethal venom, was injected in mice without lethal exits. The native venom (NAT), or its toxoided (TXD) derivative were incubated in presence or absence of mitogens, with human mononuclear (MN), B and T cells, with a pulse of [3H]Thymidine. No synergistic or antagonistic effects were observed in the combined activity of the mitogens and NAT or TXD. In the direct action of NAT the incorporation of radioactivity into MN and T cells diminished with venom increase in concentration indicating that the cytotoxicity of the native venom was correlated with the amount added. With B cells, the native venom exercised an initial mitogenic activity, declining in the higher concentration. On the other hand, the TXD showed a consistent effect, increasing the thymidine uptake in a manner related to concentration. This stimulation by TXD was observed with all groups of cells. The results indicate that, by abolishing direct cytotoxic activity with toxoiding of this venom, a derivative that enhances mitogenesis in these white cells can be obtained.
通过逐步掺入稳定的阳离子碘，对杜氏响尾蛇（Crotalus durissus terrificus）的毒液进行了解毒。将相当于100个致死毒液半数致死量（LD50）剂量的类毒素注射到无致死风险的小鼠体内。将天然毒液（NAT）或其类毒素衍生物（TXD）在有或无丝裂原存在的情况下，与人单核细胞（MN）、B细胞和T细胞一起孵育，并加入[3H]胸腺嘧啶核苷脉冲。在丝裂原与NAT或TXD的联合活性中未观察到协同或拮抗作用。在NAT的直接作用中，随着毒液浓度增加，MN和T细胞中放射性的掺入减少，这表明天然毒液的细胞毒性与添加量相关。对于B细胞，天然毒液最初具有促有丝分裂活性，在较高浓度时活性下降。另一方面，TXD显示出一致的效果，以与浓度相关的方式增加胸腺嘧啶核苷摄取。在所有细胞组中均观察到TXD的这种刺激作用。结果表明，通过对这种毒液进行类毒素处理消除其直接细胞毒性，可以获得一种能增强这些白细胞有丝分裂的衍生物。
