Guttormsen H K, Baker C J, Edwards M S, Paoletti L C, Kasper D L
Channing Laboratory, Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115, USA.
J Infect Dis. 1996 Jan;173(1):142-50. doi: 10.1093/infdis/173.1.142.
The susceptibility of neonates to invasive infection with type III group B streptococci (GBS) in a radioactive antigen-binding assay (RABA) has been correlated with low maternal serum levels of capsular polysaccharide-specific antibodies. An ELISA was developed using capsular polysaccharide covalently coupled to human serum albumin. In sera from 35 healthy women, the range of IgG antibodies to GBS III polysaccharide was 0.05-33.0 micrograms/mL, and specific IgA antibodies were 0.08-1.1 micrograms/mL; however, no GBS III capsular polysaccharide-specific antibodies of the IgM isotype were detected by the ELISA. The levels of naturally acquired and vaccine-induced antibodies obtained with this ELISA correlated well with the results of the RABA (Spearman's rank correlation coefficient, .92). The ELISA has two major advantages over the RABA: It measures specific isotypes and subclasses of antibodies, and it can detect type III polysaccharide-specific antibodies at lower concentrations.
在放射性抗原结合试验（RABA）中，新生儿对B族链球菌III型（GBS）侵袭性感染的易感性与母体血清中荚膜多糖特异性抗体水平较低相关。利用与人类血清白蛋白共价偶联的荚膜多糖开发了一种酶联免疫吸附测定（ELISA）。在35名健康女性的血清中，GBS III多糖的IgG抗体范围为0.05 - 33.0微克/毫升，特异性IgA抗体为0.08 - 1.1微克/毫升；然而，ELISA未检测到IgM同种型的GBS III荚膜多糖特异性抗体。用该ELISA获得的自然获得性抗体和疫苗诱导抗体水平与RABA结果高度相关（斯皮尔曼等级相关系数为0.92）。与RABA相比，ELISA有两个主要优点：它可测量抗体的特异性同种型和亚类，并且能够检测更低浓度的III型多糖特异性抗体。
