Mikloska Z, Kesson A M, Penfold M E, Cunningham A L
Virology Department, Westmead Hospital, Australia.
J Infect Dis. 1996 Jan;173(1):7-17. doi: 10.1093/infdis/173.1.7.
In early recurrent herpetic lesions, CD4 T lymphocytes are the predominant infiltrating cells, and keratinocytes expressing major histocompatibility complex (MHC) class II antigens, induced by interferon-gamma (IFN-gamma), are the major site of herpes simplex virus (HSV) replication. IFN-gamma pretreatment of human keratinocytes in vitro reduced MHC class I antigen down-regulation by HSV-1 infection and induced expression of HLA-DR that was unaltered by subsequent HSV-1 infection. Incubation of these infected keratinocytes with phosphonoacetic acid (PAA) almost completely inhibited expression of four major HSV glycoproteins, although expression of early proteins was not affected. Weak CD8 T lymphocyte cytotoxicity against IFN-gamma-stimulated, HLA-DR-expressing HSV-1-infected keratinocytes was consistently directed to the immediate early/early proteins (all 9 patients tested) but against late proteins to a lesser degree (4/9 patients). However, CD4 T lymphocyte cytotoxicity was much greater and directed predominantly against late HSV-1 glycoproteins (all 9 subjects tested) in these cells.
在早期复发性疱疹性损害中，CD4 T淋巴细胞是主要的浸润细胞，而由γ干扰素（IFN-γ）诱导表达主要组织相容性复合体（MHC）Ⅱ类抗原的角质形成细胞是单纯疱疹病毒（HSV）复制的主要部位。体外对人角质形成细胞进行IFN-γ预处理可减少HSV-1感染导致的MHCⅠ类抗原下调，并诱导HLA-DR表达，后续HSV-1感染对此表达无影响。用膦甲酸（PAA）孵育这些被感染的角质形成细胞几乎完全抑制了四种主要HSV糖蛋白的表达，尽管早期蛋白的表达未受影响。针对IFN-γ刺激的、表达HLA-DR的HSV-1感染角质形成细胞的CD8 T淋巴细胞细胞毒性始终针对即刻早期/早期蛋白（所有9例受试患者），但针对晚期蛋白的程度较轻（4/9例患者）。然而，在这些细胞中，CD4 T淋巴细胞细胞毒性要强得多，且主要针对晚期HSV-1糖蛋白（所有9例受试对象）。
