Takimoto M, Kuzumaki N
Laboratory of Molecular Genetics, Hokkaido University School of Medicine.
Nihon Rinsho. 1995 Nov;53(11):2666-71.
The methods for the detection and the identification of tumor suppressor genes are characterized and classified as follows: Firstly; by the comparison of expressed genes or proteins between tumor cells and normal cells. Secondly; by the gene transfer. Thirdly; by the identification of proteins which are involved in the biochemical process for cell growth suppression and DNA repair. Human genome contains dozens of tumor suppressor genes and tumor suppression-related genes which have not yet been cloned. Positional cloning is the most reliable method for the cloning of these genes. However, it cost huge amount of labor and time. New methods developed in recent years, such as differential mRNA display or two hybrids system, would facilitate the identification and the molecular cloning of novel tumor suppressor genes.
首先，通过比较肿瘤细胞和正常细胞之间表达的基因或蛋白质；其次，通过基因转移；第三，通过鉴定参与细胞生长抑制和DNA修复生化过程的蛋白质。人类基因组包含数十个尚未克隆的肿瘤抑制基因和肿瘤抑制相关基因。定位克隆是克隆这些基因最可靠的方法。然而，这需要耗费大量的人力和时间。近年来开发的新方法，如差异mRNA显示或双杂交系统，将有助于新型肿瘤抑制基因的鉴定和分子克隆。
