Fu K, Izquierdo R, Hubbard T, Fareed J
Division of Plastic Surgery, Loyola University Chicago, USA.
Microsurgery. 1995;16(8):536-41. doi: 10.1002/micr.1920160806.
A crush-avulsion anastomosis model was established on the rat femoral vein. Saline or heparin was used as a luminal topical agent and was allowed to contact the damaged endothelium for 10 min. Patency and coagulation parameters were investigated for 1 week. The heparin treated group had a patency rate of 93% at 1 hr vs. 13% for the saline treated group (P < 0.001). At 7 days, the heparin treated group had an 87% patency vs. 7% for the saline-treated group (P < 0.001). Scanning electron micrography (SEM) provided evidence of the deposition of the components of early thrombosis in the crushed venous wall. In contrast, the SEM of the heparin treated group shows a paucity of any evidence of thrombus. These results indicate that the rat vein crush-avulsion model is a reliable and reproducible thrombosis model with low patency. The methods used with the topical agent may improve the patency rate in crush avulsion injuries.
在大鼠股静脉上建立挤压-撕脱吻合模型。使用生理盐水或肝素作为腔内局部用药，使其与受损内皮接触10分钟。对通畅率和凝血参数进行为期1周的研究。肝素治疗组在1小时时的通畅率为93%，而生理盐水治疗组为13%（P<0.001）。在7天时，肝素治疗组的通畅率为87%，生理盐水治疗组为7%（P<0.001）。扫描电子显微镜（SEM）提供了早期血栓成分在挤压静脉壁沉积的证据。相比之下，肝素治疗组的SEM显示几乎没有血栓形成的证据。这些结果表明，大鼠静脉挤压-撕脱模型是一种可靠且可重复的低通畅率血栓形成模型。局部用药所采用的方法可能会提高挤压撕脱伤的通畅率。
