O'Dea K P, McKean P G, Harris A, Brown K N
Division of Parasitology, National Institute for Medical Research, Mill Hill, London, UK.
Mol Biochem Parasitol. 1995 Jun;72(1-2):111-9. doi: 10.1016/0166-6851(95)00090-n.
Processing of the Plasmodium merozoite surface protein 1 (MSP-1) has been described for parasites maintained under in vitro conditions. We have now demonstrated, using CBA/Ca mice infected with Plasmodium chabaudi chabaudi AS, that MSP-1 processing also occurs in vivo. The major proteolytic cleavage sites and a processing scheme were deduced from N-terminal amino-acid sequences of the MSP-1 breakdown products. Comparison of MSP-1 processing in P. falciparum and P.c. chabaudi indicates a degree of conservation and in two cases the position of protease cleavage appears identical. Significant amounts of MSP-1 polypeptides are found in plasma during schizogony. Various aspects of MSP-1 processing including immunological and physiological reactions in the host during the critical period of schizogony can now be examined in vivo.
疟原虫裂殖子表面蛋白1（MSP-1）的加工过程已在体外培养的寄生虫中得到描述。我们现在利用感染了恰氏疟原虫阿氏亚种的CBA/Ca小鼠证明，MSP-1的加工过程也发生在体内。从MSP-1降解产物的N端氨基酸序列推导出了主要的蛋白水解切割位点和加工方案。恶性疟原虫和恰氏疟原虫中MSP-1加工过程的比较表明存在一定程度的保守性，在两个案例中蛋白酶切割的位置似乎相同。在裂体增殖期间，血浆中发现了大量的MSP-1多肽。现在可以在体内研究MSP-1加工过程的各个方面，包括裂体增殖关键期宿主中的免疫和生理反应。
