通过原位杂交检测到的一种新型恶性疟原虫基因的性阶段特异性RNA表达。

Sexual-stage-specific RNA expression of a new Plasmodium falciparum gene detected by in situ hybridisation.

作者信息

Baker D A, Thompson J, Daramola O O, Carlton J M, Targett G A

机构信息

Department of Medical Parasitology, London School of Hygiene and Tropical Medicine, UK.

出版信息

Mol Biochem Parasitol. 1995 Jun;72(1-2):193-201. doi: 10.1016/0166-6851(95)00073-a.

DOI:10.1016/0166-6851(95)00073-a

PMID:8538689

Abstract

A gene, Pf77, transcribed in the sexual stages of Plasmodium falciparum was isolated from a genomic expression library with a polyclonal antibody raised to gametocyte proteins. The entire coding region was obtained from a series of overlapping genomic and cDNA clones (from gametocyte RNA). A single open reading frame is present with no introns and no tandem repeat sequences. A Pf77 probe hybridised to a single transcript present in RNA prepared from purified gametocytes and could not be detected in RNA prepared from asexual blood stages. In situ hybridisation studies confirmed that the expression of Pf77 mRNA is sexual-stage-specific and in addition, showed that Pf77 mRNA is present only in female gametocytes during the vertebrate stages of the parasite's development.

摘要

从一个基因组表达文库中，利用针对配子体蛋白产生的多克隆抗体，分离出了在恶性疟原虫有性阶段转录的一个基因Pf77。整个编码区是从一系列重叠的基因组和cDNA克隆（来自配子体RNA）中获得的。存在一个单一的开放阅读框，没有内含子和串联重复序列。Pf77探针与从纯化的配子体制备的RNA中存在的单一转录本杂交，而在从无性血液阶段制备的RNA中未检测到。原位杂交研究证实，Pf77 mRNA的表达是有性阶段特异性的，此外，还表明在寄生虫发育的脊椎动物阶段，Pf77 mRNA仅存在于雌配子体中。