DNA拓扑异构酶II的结构与机制。
Structure and mechanism of DNA topoisomerase II.
作者信息
Berger J M, Gamblin S J, Harrison S C, Wang J C
机构信息
Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
出版信息
Nature. 1996 Jan 18;379(6562):225-32. doi: 10.1038/379225a0.
The crystal structure of a large fragment of yeast type II DNA topoisomerase reveals a heart-shaped dimeric protein with a large central hole. It provides a molecular model of the enzyme as an ATP-modulated clamp with two sets of jaws at opposite ends, connected by multiple joints. An enzyme with bound DNA can admit a second DNA duplex through one set of jaws, transport it through the cleaved first duplex, and expel it through the other set of jaws.
酵母II型DNA拓扑异构酶大片段的晶体结构揭示了一种心形二聚体蛋白，其中心有一个大孔。它提供了该酶的分子模型，即一种由ATP调节的夹子，在相对两端有两组钳口，通过多个关节相连。结合了DNA的酶可以通过一组钳口接纳第二条DNA双链，使其穿过被切割的第一条双链，然后通过另一组钳口将其排出。
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