Kippersund J T, Haukeland B, Lilleeng B F, Daehlin L, Haukaas S, Høisaeter P A, Ogreid D
Senter for klinisk molekylaermedisin, Haukeland Sykehus, Bergen.
Tidsskr Nor Laegeforen. 1995 Nov 30;115(29):3612-5.
Prostate cancer is the most common malignant disease in men in western societies. Extracapsular spread of carcinoma is found in approximately half of the patients that are treated by radical prostatectomy. Recently, a new prostate-specific membrane glycoprotein was cloned and sequenced. A highly sensitive and specific nested reverse transcriptase polymerase chain reaction has been developed to detect early occult haematogeneous micrometastatic prostate cells. We analysed venous samples from 17 patients with metastatic prostate cancer using a modified reaction assay. This showed presence of micrometastatic prostate cells in 14 patients. Molecular detection of circulating prostatic epithelial cells could improve clinical staging and treatment of early prostate cancer.
前列腺癌是西方社会男性中最常见的恶性疾病。在接受根治性前列腺切除术治疗的患者中，约有一半发现癌组织有包膜外扩散。最近，一种新的前列腺特异性膜糖蛋白被克隆并测序。已开发出一种高度灵敏且特异的巢式逆转录聚合酶链反应来检测早期隐匿性血行微转移前列腺细胞。我们使用改良反应分析法分析了17例转移性前列腺癌患者的静脉样本。结果显示，14例患者存在微转移前列腺细胞。循环前列腺上皮细胞的分子检测可改善早期前列腺癌的临床分期和治疗。
