Green M D, Mount D L, Todd G D
Entomology Branch, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
Analyst. 1995 Oct;120(10):2623-6. doi: 10.1039/an9952002623.
A simple method is described for the extraction and subsequent analysis of sulfadoxine in human whole blood using a solid-phase extraction technique and colorimetric reaction. This procedure utilizes the micellar properties of sodium dodecyl sulfate to: (1) extract sulfadoxine from a C18 solid-phase sample-preparation column; (2) enhance the colorimetric reaction produced by the addition of p-dimethylaminocinnamaldehyde (DMAC); and (3) provide stability to the coloured product generated by the reaction of sulfadoxine with DMAC. The intense, violet-red colour reaction can be conveniently used for qualitative and semiquantitative visual interpretations of sulfadoxine levels. Under the assay conditions, drug concentrations in the blood of subjects receiving sulfadoxine were determined from absorbance measurements. These results correlated well with the sulfadoxine levels determined from high-performance liquid chromatographic analysis. Important advantages of the procedure include the ability to evaluate small samples of whole blood (100 microliters), the minimal use of organic solvents, no sophisticated instrumentation, and formation of a stable, coloured reaction product. The method proved to be a suitable field assay for determining whole-blood levels of sulfonamides in the concentration range from 5 to 100 micrograms ml-1.
本文描述了一种使用固相萃取技术和比色反应从人全血中提取并随后分析磺胺多辛的简单方法。该方法利用十二烷基硫酸钠的胶束特性：(1) 从C18固相样品制备柱中提取磺胺多辛；(2) 增强加入对二甲氨基肉桂醛（DMAC）产生的比色反应；(3) 为磺胺多辛与DMAC反应生成的有色产物提供稳定性。强烈的紫红色显色反应可方便地用于磺胺多辛水平的定性和半定量视觉解读。在测定条件下，通过吸光度测量确定接受磺胺多辛治疗的受试者血液中的药物浓度。这些结果与通过高效液相色谱分析测定的磺胺多辛水平相关性良好。该方法的重要优点包括能够评估小体积的全血样本（100微升）、有机溶剂使用量最少、无需复杂仪器，以及形成稳定的有色反应产物。该方法被证明是一种适用于测定浓度范围为5至100微克/毫升的全血中磺胺类药物水平的现场检测方法。
