Richter E, Greinert U, Kirsten D, Rüsch-Gerdes S, Schlüter C, Duchrow M, Galle J, Magnussen H, Schlaak M, Flad H D, Gerdes J
Department of Immunology and Cell Biology, Forschungsinstitut Borstel, Germany.
Am J Respir Crit Care Med. 1996 Jan;153(1):375-80. doi: 10.1164/ajrccm.153.1.8542146.
In this study we applied a polymerase chain reaction (PCR) assay for the detection and species-specific identification of mycobacteria to samples from patients with sarcoidosis and mycobacterial infections and from control patients. The PCR-technique is based on the amplification of mycobacterial DNA coding for 16S rRNA, which is present in all mycobacterial species, and on the additional sequencing of the PCR fragment to determine the species. Mycobacterial DNA could be detected in lung tissues and bronchoalveolar lavage cells from cases of tuberculosis and infections with atypical mycobacteria. On the other hand, mycobacterial DNA was amplified only in lung tissue from one patient with sarcoidosis. Twenty-three samples from patients with sarcoidosis were negative for mycobacterial DNA. From our results we conclude that the granulomatous lesions in sarcoidosis may not be due to mycobacterial infections.
在本研究中，我们将一种用于检测分枝杆菌并进行种属特异性鉴定的聚合酶链反应（PCR）检测方法应用于结节病患者、分枝杆菌感染患者以及对照患者的样本。PCR技术基于对编码16S rRNA的分枝杆菌DNA进行扩增（所有分枝杆菌种属中均存在该DNA），并对PCR片段进行额外测序以确定种属。在肺结核病例和非典型分枝杆菌感染病例的肺组织及支气管肺泡灌洗细胞中可检测到分枝杆菌DNA。另一方面，仅在一名结节病患者的肺组织中扩增出了分枝杆菌DNA。23份结节病患者的样本分枝杆菌DNA检测呈阴性。根据我们的结果，我们得出结论，结节病中的肉芽肿性病变可能并非由分枝杆菌感染所致。
