Le Minor S, Pigache F
Ann Microbiol (Paris). 1978 Oct;129 B(3):407-23.
The somatic O-antigens of 583 French isolates of Serratia marcescens were studied with the aid of the tube O-agglutination technique sensitized by centrifugation. Flagella antigens were identified by the H-immobilization test. Five new O-antigens (O16 to O20) were found. An antigenic factor (Co) common to isolates of serotypes O12:H9, O14:H12, O14:H20, and O13:H17 is described. Agar cultures of serotypes O12:H9 and O14:H12 dissociated into two colonial types: iridescent colonies, and non-iridescent colonies. Non-iridescent colonies, heated at 100 degrees C, are agglutinated by anti-Co serum, whereas heat-treated iridescent colonies are not. Close relationship between serogroup antigens O6 and O14 observed by traub and Kleber is confirmed. Serogroup O9 was subdivided into two subgroups. In France, 49 different serotypes were identified, with a predominance of serotypes O14:H12, O13:H17 and O3:H12. Recently, several new H-antigens were independently described by Traub and Kleber, and by us. To avoid confusion, these new H-antigens have been herein redesignated in accord with Dr Traub.
借助经离心致敏的试管O凝集技术,对583株法国粘质沙雷氏菌分离株的体细胞O抗原进行了研究。通过H固定试验鉴定鞭毛抗原。发现了5种新的O抗原(O16至O20)。描述了O12:H9、O14:H12、O14:H20和O13:H17血清型分离株共有的一种抗原因子(Co)。O12:H9和O14:H12血清型的琼脂培养物解离为两种菌落类型:彩虹色菌落和非彩虹色菌落。加热至100℃的非彩虹色菌落可被抗Co血清凝集,而热处理的彩虹色菌落则不能。证实了Traub和Kleber观察到的O6和O14血清群抗原之间的密切关系。O9血清群被细分为两个亚群。在法国,鉴定出49种不同的血清型,其中以O14:H12、O13:H17和O3:H12血清型为主。最近,Traub和Kleber以及我们分别独立描述了几种新的H抗原。为避免混淆,本文根据Traub博士的意见对这些新的H抗原进行了重新命名。