Lidow M S, Rakic P
Yale University School of Medicine, Section of Neurobiology, New Haven, Connecticut 06510, USA.
J Comp Neurol. 1995 Sep 25;360(3):393-402. doi: 10.1002/cne.903600303.
Film autoradiography was used to investigate the expression of several neurotransmitter receptor subtypes in the transient ventricular and subventricular proliferative zones of the developing occipital lobe in two groups of macaque monkey fetuses. The first group of fetuses were between 60 and 93 days after conception (E60-E93), when the ventricular and subventricular zones of the monkey occipital lobe produce neurons destined for the visual cortex. In the second group, fetuses were between E107 and E128, after generation of cortical neurons has ceased. In the E60-E93 group of fetuses, ventricular and subventricular zones displayed high densities of 5-HT1-serotonergic, D1-dopaminergic, alpha 1- and alpha 2-adrenergic and high affinity kainate receptors. The activation of these receptors has previously been shown to stimulate cell proliferation in other cell systems. The possible involvement of these receptors in regulation of neuronal production is also supported by their absence in the deep laminae of the embryonic cerebral wall after E107, after cortical neurogenesis has been completed. The only exception is a high density of alpha 2-adrenergic receptors maintained near the ventricular surface long after all cortical neurons have been generated. We also found that during neurogenesis, proliferative zones in E66-E90 fetuses displayed virtually no 5-HT2-serotonergic, D2-dopaminergic, beta-adrenergic, M1-muscarinic cholinergic, gamma aminobutyric acid (GABA)A, N-methyl-D-aspartate (NMDA), or alpha-amino-3-hydroxy-5-menthy-4-isoxazole proprionate (AMPA) sites; most of these receptor subtypes have been reported to mediate the suppression of cell proliferation. The present findings suggest that dividing and/or newly generated cortical neurons are capable of receiving specific signals from multiple neurotransmitters present in their environment.
运用放射自显影片技术，对两组猕猴胎儿发育中的枕叶瞬时脑室和脑室下增殖区中几种神经递质受体亚型的表达进行了研究。第一组胎儿处于受孕后60至93天（E60 - E93），此时猕猴枕叶的脑室和脑室下区产生 destined for the visual cortex 的神经元。第二组胎儿处于E107至E128，此时皮质神经元的产生已经停止。在E60 - E93组胎儿中，脑室和脑室下区显示出高密度的5 - HT1 - 血清素能、D1 - 多巴胺能、α1 - 和α2 - 肾上腺素能以及高亲和力的海人藻酸受体。先前已表明这些受体的激活会刺激其他细胞系统中的细胞增殖。在E107之后，即皮质神经发生完成后，胚胎脑壁深层板层中这些受体的缺失也支持了它们可能参与神经元生成调节的观点。唯一的例外是，在所有皮质神经元产生很久之后，脑室表面附近仍维持着高密度的α2 - 肾上腺素能受体。我们还发现，在神经发生过程中，E66 - E90胎儿的增殖区几乎没有5 - HT2 - 血清素能、D2 - 多巴胺能、β - 肾上腺素能、M1 - 毒蕈碱胆碱能、γ - 氨基丁酸（GABA）A、N - 甲基 - D - 天冬氨酸（NMDA）或α - 氨基 - 3 - 羟基 - 5 - 甲基 - 4 - 异恶唑丙酸（AMPA）位点；据报道，这些受体亚型中的大多数介导细胞增殖的抑制。目前的研究结果表明，正在分裂和/或新产生的皮质神经元能够接收来自其周围环境中多种神经递质的特定信号。
