对自然感染犬瘟热病毒的犬类淋巴器官进行免疫组织化学分析。
Immunohistochemical analysis of the lymphoid organs of dogs naturally infected with canine distemper virus.
作者信息
Iwatsuki K, Okita M, Ochikubo F, Gemma T, Shin Y S, Miyashita N, Mikami T, Kai C
机构信息
Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
出版信息
J Comp Pathol. 1995 Aug;113(2):185-90. doi: 10.1016/s0021-9975(05)80033-7.
The pathogenesis of acute canine distemper in three naturally infected dogs was investigated. The lymphoid organs showed atrophy without secondary follicles. The distribution of canine distemper virus (CDV) antigens was examined immunohistochemically with monoclonal antibodies specific for canine Thy-1, immunoglobulin (Ig) M, CD4, CD8, CD21 and CD45RB, and anti-measles virus nucleocapsid protein serum. The viral antigens were located in the T-cell-dependent areas and in the follicles of lymphoid organs; they were observed mainly in the Thy-1, or CD4-positive cells, but also in the CD8-, CD21-, or IgM-positive cells. The results indicated that Thy-1-positive and CD4-positive T cells serve as major target cells for CDV during the acute stage of infection.
对三只自然感染犬瘟热的犬的急性犬瘟热发病机制进行了研究。淋巴器官显示萎缩且无次级滤泡。用针对犬Thy-1、免疫球蛋白（Ig）M、CD4、CD8、CD21和CD45RB的单克隆抗体以及抗麻疹病毒核衣壳蛋白血清，通过免疫组织化学方法检测犬瘟热病毒（CDV）抗原的分布。病毒抗原位于淋巴器官的T细胞依赖区和滤泡中；主要在Thy-1或CD4阳性细胞中观察到，但也在CD8、CD21或IgM阳性细胞中观察到。结果表明，在感染急性期，Thy-1阳性和CD4阳性T细胞是CDV的主要靶细胞。
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