Division of Zoonoses, Institute of Special Economic Animal and Plant Sciences, State Key Laboratory of Special Economic Animal Molecular Biology, Chinese Academy of Agricultural Sciences CAAS, 15 Luming Street, Jilin 132109, China.
Virol J. 2011 Nov 16;8:520. doi: 10.1186/1743-422X-8-520.
A new isolate of canine distemper virus (CDV), named ZJ7, was isolated from lung tissues of a dog suspected with CDV infection using MDCK cells. The ZJ7 isolate induced cytopathogenic effects of syncytia in MDCK cell after six passages. In order to evaluate pathogenesis of ZJ7 strain, three CDV sero-negative dogs were intranasally inoculated with its virus suspension. All infected dogs developed clinical signs of severe bloody diarrhea, conjunctivitis, ocular discharge, nasal discharge and coughing, fever and weight loss at 21 dpi, whereas the mock group infected with DMEM were normal. The results demonstrated that CDV-ZJ7 strain isolated by MDCK cell was virulent, and the nucleotide and amino acid sequences of strain ZJ7 had no change after isolation by MDCK cell when compared with the original virus from the fresh tissues. Molecular and phylogenetic analyses for the nucleocapsid (N), phosphoprotein (P) and receptor binding haemagglutinin (H) gene of the ZJ7 isolate clearly showed it is joins to the Asia 1 group cluster of CDV strains, the predominant genotype in China.
一株新的犬瘟热病毒（CDV）分离株 ZJ7 是从疑似感染 CDV 的犬的肺组织中，通过 MDCK 细胞分离得到的。ZJ7 分离株在 MDCK 细胞中连续传代 6 次后，诱导出合胞体的细胞病变效应。为了评估 ZJ7 株的致病力，将其病毒悬液滴鼻接种 3 只 CDV 血清阴性犬。所有感染犬在 21dpi 时均出现严重血样腹泻、结膜炎、眼分泌物、鼻分泌物和咳嗽、发热和体重减轻的临床症状，而用 DMEM 感染的模拟组则正常。结果表明，用 MDCK 细胞分离的 CDV-ZJ7 株是毒力株，与从新鲜组织中分离的原始病毒相比，ZJ7 株在 MDCK 细胞中分离后，其核苷酸和氨基酸序列没有变化。对 ZJ7 分离株的核衣壳（N）、磷蛋白（P）和受体结合血凝素（H）基因进行的分子和系统进化分析表明，它属于中国主要流行基因型的 CDV 亚洲 1 群簇。
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