Terada N, Konno A, Terada Y, Fukuda S, Yamashita T, Abe T, Shimada H, Ishida K, Yoshimura K, Tanaka Y
Department of Otorhinolaryngology, Chiba University, School of Medicine, Japan.
J Allergy Clin Immunol. 1995 Dec;96(6 Pt 2):1161-9. doi: 10.1016/s0091-6749(95)70201-6.
By using the reverse transcription polymerase chain reaction and Southern blot hybridization, we demonstrated that Fc epsilon RI alpha-chain (Fc epsilon RI alpha) messenger RNA was expressed in eosinophils purified from the peripheral blood of patients with allergic rhinitis and that this expression was enhanced by IL-4. However, studies in which flow cytometry or immunostaining was used did not reveal the expression of Fc epsilon RI alpha protein on eosinophils from peripheral blood. Neither IL-4 alone nor the combination of IL-4 and other cytokines could induce detectable Fc epsilon RI alpha protein; nevertheless, they do express Fc epsilon RI alpha mRNA. Double-labeling immunostaining on cryostat sections of nasal mucosa clearly demonstrated that some Fc epsilon RI alpha-positive cells were eosinophil cationic protein-positive, which confirms their eosinophilic nature. Not all the eosinophil cationic protein-positive cells express on Fc epsilon RI alpha signal. Considering that Fc epsilon RI alpha mRNA was detectable in four of five samples of eosinophils from those patients with nasal allergy and that only one of five eosinophil samples from normal subjects expressed Fc epsilon RI alpha mRNA, the level of Fc epsilon RI expression may be correlated with the activation of eosinophils. It seems very likely that some other unidentified factors are required for the process from the expression of Fc epsilon RI alpha mRNA to that of Fc epsilon RI as a protein.
通过逆转录聚合酶链反应和Southern印迹杂交，我们证明了FcεRIα链（FcεRIα）信使核糖核酸在从变应性鼻炎患者外周血中纯化的嗜酸性粒细胞中表达，并且这种表达被白细胞介素-4增强。然而，使用流式细胞术或免疫染色的研究未显示外周血嗜酸性粒细胞上存在FcεRIα蛋白表达。单独的白细胞介素-4或白细胞介素-4与其他细胞因子的组合均不能诱导可检测到的FcεRIα蛋白；尽管如此，它们确实表达FcεRIα信使核糖核酸。对鼻黏膜冰冻切片进行双标记免疫染色清楚地表明，一些FcεRIα阳性细胞是嗜酸性粒细胞阳离子蛋白阳性，这证实了它们的嗜酸性本质。并非所有嗜酸性粒细胞阳离子蛋白阳性细胞都表达FcεRIα信号。鉴于在五分之四的鼻过敏患者的嗜酸性粒细胞样本中可检测到FcεRIα信使核糖核酸，而在正常受试者的五分之一嗜酸性粒细胞样本中只有一个表达FcεRIα信使核糖核酸，FcεRI的表达水平可能与嗜酸性粒细胞的活化相关。从FcεRIα信使核糖核酸的表达到作为蛋白质的FcεRI的表达过程似乎很可能还需要一些其他未确定的因素。
