凝固酶阳性葡萄球菌过氧化氢酶的比较免疫学研究

A comparative immunological study of catalases from coagulase-positive staphylococci.

作者信息

Rupprecht M, Schleifer K H

出版信息

Arch Microbiol. 1979 Jan 16;120(1):53-6. doi: 10.1007/BF00413273.

Abstract

Protein homology studies with catalase as a reference point were carried out with coagulase-positive staphylococci belonging to Staphylococcus aureus, S. intermedius and S. hyicus. Antisera against catalases of S. aureus ATCC 12600 and S. aureus ATCC12601 reacted very weakly employing double immunodiffusion and quantitative microcomplement-fixation assay with cell-free extracts or catalase enriched preparations of S. intermedius or S. hyicus. The differences between coagulase-positive staphylococci could be confirmed by using the antiserum against S. intermedius H 11 catalase. Within the strains of the species S. intermedius immunological distances ranging up to 25 indicate a heterogeneity which justify the separation of the biotypes E and F on a subspecies level. Coagulase-positive strains of S. hyicus revealed neither a close relationship to S. aureus nor to S. intermedius.

摘要

以过氧化氢酶为参照点，对属于金黄色葡萄球菌、中间葡萄球菌和猪葡萄球菌的凝固酶阳性葡萄球菌进行了蛋白质同源性研究。采用双向免疫扩散和定量微量补体结合试验，以中间葡萄球菌或猪葡萄球菌的无细胞提取物或富含过氧化氢酶的制剂为抗原，抗金黄色葡萄球菌ATCC 12600和金黄色葡萄球菌ATCC12601过氧化氢酶的抗血清反应非常微弱。使用抗中间葡萄球菌H 11过氧化氢酶的抗血清可以证实凝固酶阳性葡萄球菌之间的差异。在中间葡萄球菌的菌株中，免疫距离高达25表明存在异质性，这证明在亚种水平上分离生物型E和F是合理的。猪葡萄球菌的凝固酶阳性菌株与金黄色葡萄球菌和中间葡萄球菌均无密切关系。