Molnár A, Wu P, Largespada D A, Vortkamp A, Scherer S, Copeland N G, Jenkins N A, Bruns G, Georgopoulos K
Cutaneous Biology Research Center, Massachusetts General Hospital, Boston, MA 02129, USA.
J Immunol. 1996 Jan 15;156(2):585-92.
The Ikaros gene is an essential regulator in the development and homeostasis of the mouse lymphopoietic system. To study the role of the Ikaros gene in the human lymphopoietic system, we cloned and characterized human Ikaros cDNAs. In the human, as in the mouse, differential splicing of Ikaros primary transcripts generates a family of lymphoid-restricted zinc finger DNA binding proteins, highly conserved in sequence composition and relative expression to the mouse homologues. Expression of Ikaros isoforms is highly restricted to the lymphopoietic system and is particularly enriched in maturing thymocytes. The Ikaros gene maps at a syntenic locus located on the short arm of human chromosome 7 and on mouse chromosome 11 next to the epidermal growth factor receptor (Egfr). The high degree of conservation of the Ikaros gene at the genetic and expression levels strongly suggests that it plays a fundamental role in the ontogeny of the lymphopoietic system across species.
Ikaros基因是小鼠淋巴细胞生成系统发育和稳态的重要调节因子。为了研究Ikaros基因在人类淋巴细胞生成系统中的作用，我们克隆并鉴定了人类Ikaros cDNA。在人类中，如同在小鼠中一样，Ikaros初级转录本的可变剪接产生了一系列淋巴特异性锌指DNA结合蛋白，其序列组成和相对于小鼠同源物的相对表达高度保守。Ikaros异构体的表达高度局限于淋巴细胞生成系统，在成熟胸腺细胞中尤其丰富。Ikaros基因定位于人类7号染色体短臂和小鼠11号染色体上与表皮生长因子受体（Egfr）相邻的同线位点。Ikaros基因在遗传和表达水平上的高度保守强烈表明，它在跨物种淋巴细胞生成系统的个体发生中起着基本作用。
