Haynes K A, Westerneng T J, Fell J W, Moens W
Department of Medical Microbiology, Charing Cross & Westminster Medical School, London, UK.
J Med Vet Mycol. 1995 Sep-Oct;33(5):319-25. doi: 10.1080/02681219580000641.
We describe a polymerase chain reaction (PCR) based approach to the detection and identification of pathogenic fungi which has potential for the diagnosis of systemic mycoses. Primers to sequences of the large subunit ribosomal DNA genes, which are universally conserved within the fungal kingdom, were capable of amplifying DNA from 43 strains representing 20 species (12 genera) of medically important fungi. Sequence analysis of the products obtained from Aspergillus fumigatus, Candida albicans and Cryptococcus neoformans allowed us to design species-specific primers which only amplified homologous DNA. The use of these two PCRs in tandem allows the detection (universal PCR) and identification (species-specific PCR) of a fungal pathogen within 8 h from simulated clinical specimens.
我们描述了一种基于聚合酶链反应（PCR）的方法，用于检测和鉴定致病真菌，该方法在系统性真菌病的诊断中具有潜力。针对核糖体大亚基DNA基因序列的引物在真菌界普遍保守，能够扩增来自代表20种（12属）医学上重要真菌的43株菌株的DNA。对烟曲霉、白色念珠菌和新型隐球菌获得的产物进行序列分析，使我们能够设计出仅扩增同源DNA的种特异性引物。串联使用这两种PCR能够在8小时内从模拟临床标本中检测（通用PCR）和鉴定（种特异性PCR）真菌病原体。
