Xu G P, Sharma V K, Li B, Bologa R, Li Y, Mouradian J, Wang J, Serur D, Rao V, Stenzel K H
Rogosin Institute, Department of Transplantation Medicine and Extracorporeal Therapy, New York Hospital-Cornell Medical Center, New York, USA.
Kidney Int. 1995 Nov;48(5):1504-7. doi: 10.1038/ki.1995.440.
A major conceptual advance is the formulation that type I cytokines (such as IL-2 and IFN-gamma) enhance cellular immunity and are host-protective, and that type II cytokines (such as IL-4 and IL-10) dampen cellular immunity and facilitate the progression of infection. We have explored the intragraft expression of type I and type II cytokines during human renal allograft rejection. RNA was isolated from 98 allograft biopsies, and reverse transcription-PCR was used to amplify and identify intragraft expression of mRNA encoding IL-2, IFN-gamma, IL-4, or IL-10. Intragraft expression of IL-7 mRNA and TGF-beta 1 mRNA was also investigated. Our investigation demonstrated that: (a) intragraft expression of IL-10 mRNA and IL-2 mRNA are significant correlates of acute rejection; (b) IL-4, IL-7, IFN-gamma and TGF-beta 1 mRNA expression do not correlate with acute rejection; and (c) IL-10 does not prevent in vivo expression of IFN-gamma, IL-2, IL-7, or TGF-beta 1. Our studies identify, for the first time, a significant association between intragraft IL-10 mRNA expression and acute rejection, and suggest that treatment strategies capable of constraining IL-10 expression might be of value in the prevention of acute rejection.
I型细胞因子（如白细胞介素-2和干扰素-γ）增强细胞免疫并具有宿主保护作用，而II型细胞因子（如白细胞介素-4和白细胞介素-10）抑制细胞免疫并促进感染进展。我们研究了人类肾移植排斥反应期间移植肾内I型和II型细胞因子的表达情况。从98份移植肾活检标本中提取RNA，采用逆转录-聚合酶链反应扩增并鉴定编码白细胞介素-2、干扰素-γ、白细胞介素-4或白细胞介素-10的mRNA在移植肾内的表达。同时也研究了白细胞介素-7 mRNA和转化生长因子-β1 mRNA在移植肾内的表达。我们的研究表明：（a）白细胞介素-10 mRNA和白细胞介素-2 mRNA在移植肾内的表达与急性排斥反应显著相关；（b）白细胞介素-4、白细胞介素-7、干扰素-γ和转化生长因子-β1 mRNA的表达与急性排斥反应无关；（c）白细胞介素-10不能阻止干扰素-γ、白细胞介素-2、白细胞介素-7或转化生长因子-β1在体内的表达。我们的研究首次确定了移植肾内白细胞介素-10 mRNA表达与急性排斥反应之间的显著关联，并表明能够抑制白细胞介素-10表达的治疗策略可能对预防急性排斥反应有价值。
