Sergio Pasqualini R, Quintans C, Valzacchi G R, Matarasso N, Martínez M
Halitus Instituto Médico, Buenos Aires, Argentina.
Medicina (B Aires). 1995;55(3):249-51.
In a patient with a diagnosis of congenital vas deferens agenesis we decided to carry out a microsurgical epididymal sperm aspiration in order to attempt an in vitro fertilization (IVF). Since during scrotal exploration no epididymus was found (probably as a consequence of a previous surgical biopsy) a testicle biopsy was performed. The latter yielded a few hundred sperms which were injected within the cytoplasm of his wife's oocytes (ICSI technique). Of the 18 oocytes, 3 were harmed during the procedure and 16 hours later signs of normal fertilization were detected in 6 of them, followed by the development of 4 embryos. These were transcervically transferred to the uterus, 3 in 8 cell stage and 1 in 6 cell stage; previously a 20 micron hole had been made with acid Tyrode solution in the pelucida zone of each embryo. Fifteen days later, the result of the HCG beta subunit assay was positive and a month later a single 19 mm normal embryo was detected by ultrasound echography.
对于一名被诊断为先天性输精管缺如的患者，我们决定进行显微外科附睾精子抽吸术，以尝试体外受精（IVF）。由于在阴囊探查过程中未发现附睾（可能是先前手术活检的结果），因此进行了睾丸活检。后者获取了数百个精子，并将其注射到他妻子的卵母细胞胞质内（ICSI技术）。在18个卵母细胞中，有3个在操作过程中受损，16小时后在其中6个中检测到正常受精迹象，随后有4个胚胎发育。这些胚胎经宫颈被转移到子宫，3个处于8细胞期，1个处于6细胞期；此前已用酸性台氏液在每个胚胎的透明带区域制造了一个20微米的孔。15天后，HCGβ亚基检测结果呈阳性，一个月后通过超声检查发现了一个19毫米的正常单胚胎。
