Masliah E, Mallory M, Alford M, Deteresa R, Saitoh T
University of California, San Diego, School of Medicine, Department of Neurosciences, La Jolla 92093-0624, USA.
Neurobiol Aging. 1995 Jul-Aug;16(4):549-56. doi: 10.1016/0197-4580(95)00050-o.
In the present study we observed that while platelet-derived growth factor (PDGF)-BB is exclusively expressed by neurons in the human brain, PDGF-AA is expressed in neurons and blood vessels. In Alzheimer's disease (AD), antibodies against PDGF-BB (but not PDGF-AA) recognized the neurofibrillary alterations of this disease. The levels of PDGF-BB correlated with the patterns of synaptic loss and sprouting while PDGF-AA immunostaining of the vessels was correlated with glial proliferation. Immunostaining was completely abolished when the antibodies were preincubated with their respective purified recombinant PDGF. Western blot analysis showed that antibodies against PDGF recognized a 31 kDa protein that was mildly increased in AD. These data suggest that PDGF, as well as other neurotrophic factors, play an important role in the mechanisms of neurofibrillary pathology in AD.
在本研究中，我们观察到，虽然血小板衍生生长因子（PDGF）-BB仅在人类大脑的神经元中表达，但PDGF-AA在神经元和血管中均有表达。在阿尔茨海默病（AD）中，抗PDGF-BB（而非PDGF-AA）的抗体识别出该疾病的神经原纤维改变。PDGF-BB的水平与突触丧失和发芽模式相关，而血管的PDGF-AA免疫染色与胶质细胞增殖相关。当抗体与各自纯化的重组PDGF预孵育时，免疫染色完全消失。蛋白质印迹分析表明，抗PDGF抗体识别出一种31 kDa的蛋白质，该蛋白质在AD中略有增加。这些数据表明，PDGF以及其他神经营养因子在AD的神经原纤维病理机制中起重要作用。
