Hadid R, Spinedi E, Daneva T, Grau G, Gaillard R C
Department of Medicine, University Hospital (CHUV), Lausanne, Switzerland.
Neuroendocrinology. 1995 Oct;62(4):348-55. doi: 10.1159/000127024.
It is known that in vivo administration of bacterial endotoxin activates immune cells to release cytokines, these substances in turn enhancing hypothalamo-pituitary-adrenal (HPA) axis function; additional evidence supports the existence of an immune-neuroendocrine sexual dimorphism. In the present study, we investigated: (1) the in vivo response of both the HPA and the immune systems to single and repeated endotoxin administrations in mice, and (2) whether testosterone possesses a modulatory effect on neuroendocrine-immune function under endotoxemia. For these purposes, adult male BALB/c mice were orchidectomized (Odx) or sham-operated and injected s.c., on alternate days, with either corn oil alone (Odx and Sham) or containing 20 micrograms of testosterone (Odx+T) until animals were killed. One week after surgery, different groups of mice were treated i.p. with bacterial lipopolysaccharide (LPS; 25 micrograms per mouse) in a single (day 1, D1) or repeated (at 24-hour intervals for 5 consecutive days) form. Animals were decapitated (on D1, D3 and D5 of the treatment) 2 h after the last injection of either vehicle alone or containing LPS (the two groups were run in parellel). Trunk blood was collected and the whole medial basal hypothalamus (wMBH), the anterior pituitary (AP) and adrenal glands were dissected. Plasma tumor necrosis factor-alpha (TNF alpha), ACTH and corticosterone (B) concentrations as well as wMBH CRH, AP ACTH and adrenal B contents were determined by specific assays.(ABSTRACT TRUNCATED AT 250 WORDS)
已知体内给予细菌内毒素会激活免疫细胞释放细胞因子，这些物质进而增强下丘脑 - 垂体 - 肾上腺（HPA）轴功能；更多证据支持免疫 - 神经内分泌性别差异的存在。在本研究中，我们调查了：（1）HPA和免疫系统对小鼠单次及重复给予内毒素的体内反应，以及（2）睾酮在内毒素血症情况下是否对神经内分泌 - 免疫功能具有调节作用。为此，成年雄性BALB/c小鼠接受去势（Odx）或假手术，每隔一天皮下注射单独的玉米油（Odx组和假手术组）或含20微克睾酮的玉米油（Odx + T组），直至动物被处死。术后一周，不同组小鼠腹腔注射细菌脂多糖（LPS；每只小鼠25微克），分为单次注射（第1天，D1）或重复注射（连续5天，每隔24小时注射一次）两种方式。在最后一次注射单独的溶媒或含LPS的溶媒后2小时（两组平行进行），动物被断头处死（在治疗的D1、D3和D5天）。采集躯干血，并解剖整个内侧基底下丘脑（wMBH）、垂体前叶（AP）和肾上腺。通过特定检测方法测定血浆肿瘤坏死因子 - α（TNFα）、促肾上腺皮质激素（ACTH）和皮质酮（B）浓度以及wMBH促肾上腺皮质激素释放激素（CRH）、AP促肾上腺皮质激素（ACTH）和肾上腺B含量。（摘要截取自250字）
