al Saedi S, Dean H, Dent W, Stockl E, Cronin C
Department of Paediatrics, University of Manitoba, Winnipeg, Canada.
Pediatrics. 1996 Jan;97(1):100-2.
To compare 17-hydroxyprogesterone (17-OHP) levels measured by quantitative serum radioimmunoassay (RIA), including an extraction step, and by screening fluoroimmunoassay (FIA) on blood spots in preterm infants.
Subjects were 39 healthy infants born at less than 31 weeks' gestational age. Each infant had weekly blood sampling, and RIA and FIA were performed on each sample.
Two hundred twenty-seven samples were taken at 28 to 41 weeks' postconceptional age. Mean +/- SD 17-OHP measured by RIA was 11.4 +/- 11.1 nmol/L (0.4 +/- 0.4 micrograms/dL), and decreased over time. Mean +/- SD 17-OHP measured by FIA was 38.96 +/- 37.3 nmol/L, greater than 17-OHP (RIA). Log(delta FIA-RIA) was inversely related to postconceptional age (R2 = .39).
Screening FIA of blood spots overestimates levels of 17-OHP in preterm infants and should not be used to determine the likelihood of congenital adrenal hyperplasia in this population. We have abandoned FIA screening for congenital adrenal hyperplasia in infants weighing less than 1500 g.
比较通过定量血清放射免疫分析（RIA，包括提取步骤）和通过对早产儿血斑进行荧光免疫分析（FIA）筛查所测得的17-羟孕酮（17-OHP）水平。
研究对象为39例孕龄小于31周的健康婴儿。每个婴儿每周采血一次，对每个样本进行RIA和FIA检测。
在孕龄28至41周时采集了227个样本。通过RIA测得的平均±标准差17-OHP为11.4±11.1 nmol/L（0.4±0.4μg/dL），且随时间下降。通过FIA测得的平均±标准差17-OHP为38.96±37.3 nmol/L，高于17-OHP（RIA法）。Log（FIA-RIA差值）与孕龄呈负相关（R2 = 0.39）。
对血斑进行FIA筛查会高估早产儿的17-OHP水平，不应将其用于确定该人群先天性肾上腺皮质增生的可能性。我们已放弃对体重小于1500 g婴儿进行先天性肾上腺皮质增生的FIA筛查。
