从接种疫苗的澳大利亚鸡群中分离出1型马立克氏病病毒。
Isolation of serotype 1 Marek's disease viruses from vaccinated Australian flocks.
作者信息
De Laney D B, Jones A E, Zerbes M, Tannock G A
机构信息
Department of Applied Biology and Biotechnology Royal Melbourne Institute of Technology Melbourne, Victoria, Australia.
出版信息
Vet Microbiol. 1995 Sep;46(1-3):213-9. doi: 10.1016/0378-1135(95)00085-o.
Field samples were received for Marek's disease virus (MDV) isolation from clinically affected flocks from several regions of Eastern Australia. Lymphocytes were fractionated in Ficoll-Paque and passaged once or twice in chicken embryo kidney cultures. Serotype-specific virus was detected in infected cultures by indirect immunofluorescence with monoclonal antibodies. Serotype 1 MDV was isolated from 10 flocks. In samples from 5 of these flocks, serotype 2 and 3 vaccine viruses were isolated from the same specimen. In a parallel study, plasmas obtained during lymphocyte isolation were tested for antibodies to MDVs by agar gel precipitin (AGP) tests using serotype 1 and 3 antigen extracts. No correlation was observed between the rate of virus isolation and AGP positivity. The AGP test was incapable of discriminating between the different MDV serotypes.
从澳大利亚东部几个地区临床上受感染的鸡群中采集了用于分离马立克氏病病毒（MDV）的田间样本。淋巴细胞在Ficoll-Paque中进行分级分离，并在鸡胚肾培养物中传代一或两次。用单克隆抗体通过间接免疫荧光在感染的培养物中检测血清型特异性病毒。从10个鸡群中分离出1型MDV。在其中5个鸡群的样本中，从同一标本中分离出2型和3型疫苗病毒。在一项平行研究中，使用1型和3型抗原提取物，通过琼脂凝胶沉淀素（AGP）试验检测淋巴细胞分离过程中获得的血浆中的MDV抗体。未观察到病毒分离率与AGP阳性之间的相关性。AGP试验无法区分不同的MDV血清型。
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